In vitro, ex vivo immune response test

A comprehensive study of the immune response to the test agents.

The service includes techniques:

2D and 3D cell culture in the co-culture: PBMC (B, T, monocytes) and mast cells (e.g. 3D model – pulmonary alveolar on insert, air-liquid culture);
stimulation of cells with immunogenic factors (e.g. antigens, adjuvants, microorganisms, viruses, vaccines);
analysis of the cytopathic effect with full photo documentation;
immunophenotyping of cells (e.g. analysis of population and subpopulation of CD4+, CD8+, CD19+ lymphocytes and profile of immunoglobulins, cytokines);
immunoenzymatic/immunofluorescence (ELISPOT) and genetic (RT-qPCR) analysis;
analysis of the level of pro-inflammatory gene expression and analysis of NGS data (among others RNA-seq, DNA-seq, GAM).

Keywords: 2D and 3D cultures, vaccines, drugs, cytokines, phenotyping, flow cytometry, NGS, ONT, RNASEQ, DNASEQ, elispot flow cytometry, DDPCR